Isra Medical Journal

Title:

Pattern of Culture and Sensitivity of Micro-OganismIsolated from Bile after Cholecystectomy

Authors:

Saif Abbass, Ishtiaq Ahmed

Published on:

2017-08-31

Journal Reference:

Volume 9- Issue 4 July - August 2017

Pages:

213-217

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ABSTRACT

OBJECTIVE: To analyze the pattern of different micro-organisms and their sensitivity to antibiotics in bile of patients aftercholecystectomy.

STUDY DESIGN: A Prospective analytic study

PLACE AND DURATION: Department of Surgery Al-Nafees Medical College and Hospital from 1st January, 2016 to 30th June, 2016.

METHODOLOGY: The bile from the resected specimens of gall bladder from all patients undergoing cholecystectomy collected underaseptic technique and sent for culture and sensitivity. Pattern of isolate and sensitivity to commonly use antimicrobial agents wereanalyzed.

RESULTS: A total of 150 patient underwent cholecystectomy and among them 83.34% of the bile specimen shows growth of differentmicro-organisms. E coli is the commonest organism (41.60%) followed by Klebsiella (26.40%) and Enterobacter (7.20%). Pseudomonaswas the least common isolate found in 2.40%) of the patients. Majority of organism are resistant to Amoxicillin + Clavulanic acid andAmpicillin. Regarding each organism Citrobacter is resistant to Ampicillin Enterococcus is sensitive to almost all antibiotic tested. E coliand Enterobacter are resistant to Amoxicillin+ Clavulanic acid and Ampicillin.

CONCLUSION: There is a significant high culture of micro-organisms and changing pattern in sensitivity of micro-organisms fromampicillin and amoxicillin plus Clavulanic acid to cephalosporin's, aminoglycosides and quinolones is observed.

KEY WORDS: Cholecystectomy, Bile, Micro-organisms, Culture, Sensitivity, Antibiotics